Protein G Resin

Simpson Biotech Co., Ltd offers a wide range of recombinant proteins which includes protein G resin. It is a surface protein originally found in the cell wall of the Streptococcus sp. Protein G contains three homologous domains with binding affinity to the constant region of various sources of IgGs. The albumin binding domain as well as the cell-surface binding site of protein G was removed to eliminate the nonspecific binding. Protein G has greater affinity than protein A for most mammalian IgGs, especially human IgG3 and rat IgG2a. Application: resuspend the resin slurry completely and transfer appropriate amount of resin to a new column. Allow the resin to settle down by gravity to pack the column bed. Equilibrate the column with 10 column volumes (cv) of the binding/wash buffer. Dilute the serum samples, ascite fluid or cell culture supernatant with binding/wash buffer (1 : 1, v/v). Shelf life: 12 months when stored unopened. Contact us for more information.